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Anti-Bax Recombinant Rabbit Monoclonal Antibody

应用范围: WB, IHC-P, IP, FC
产品类型: 兔单抗
保存温度: -20℃
产地: YOBIBIO
UniProt ID: Q07812
宿主: Rabbit
克隆性: Monoclonal
修饰: Unmodified
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Basic Information:

UniProt ID: Q07812

Calculated Mol Weight: 21 kDa

Host: Rabbit

Observed Mol Weight: 21 kDa

Clonality: Monoclonal

Reactivity: Human, Mouse, Rat

Source: Recombinant Rabbit mAb

Applications: WB, IHC-P, IP, FC

Isotype: Rabbit IgG

Purification: Affinity-chromatography

Modification: Unmodified

Immunogen: A synthesized peptide

Aliases: Apoptosis regulator BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4

Recommended Dilutions:  

WB 1:1000-1:2000

 

IHC-P 1:100-1:200

 

IP 1:20-1:50

 

FC 1:20-1:100

Storage: Rabbit IgG in 10mM phosphate buffered saline, pH7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.Store at +4 short term. Store at -20 long term. Avoid repeated freeze-thaw cycle.

Background: Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

Western blot analysis of Bax in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.

For Research Use Only. Not for Use in Diagnostic Procedures. 

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