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Anti-Cyclin D1 Recombinant Rabbit Monoclonal Antibody

应用范围: WB, IHC-P, IHC-F, ICC/IF, IP
产品类型: 兔单抗
保存温度: -20℃
产地: YOBIBIO
UniProt ID: P24385
宿主: Rabbit
克隆性: Monoclonal
修饰: Unmodified
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Basic Information:

UniProt ID: P24385

Calculated Mol Weight: 34 kDa

Host: Rabbit

Observed Mol Weight: 36 kDa

Clonality: Monoclonal

Reactivity: Human, Mouse, Rat

Source: Recombinant Rabbit mAb

Applications: WB, IHC-P, IHC-F, ICC/IF, IP

Isotype: Rabbit IgG

Purification: Affinity-chromatography

Modification: Unmodified

Immunogen: A synthesized peptide

Aliases: CCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1

Recommended Dilutions:  

WB 1:1000-1:2000

 

IHC-P 1:100-1:200

 

IHC-F 1:50-1:200

 

ICC/IF 1:50-1:200

 

IP 1:20-1:50

Storage: Rabbit IgG in 10mM phosphate buffered saline, pH7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. Store at +4 short term. Store at -20 long term. Avoid repeated freeze-thaw cycle.

Background: Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

Western blot analysis of Cyclin D1 expression in (1) MCF-7 cell lysates; (2) LnCaP cell lysates.

For Research Use Only. Not for Use in Diagnostic Procedures. 

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保存温度:-20℃
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